Chapter title |
Live-Cell Imaging of Phagosome Motility in Primary Mouse RPE Cells
|
---|---|
Chapter number | 100 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-17121-0_100 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
|
Authors |
Roni Hazim, Mei Jiang, Julian Esteve-Rudd, Tanja Diemer, Vanda S. Lopes, David S. Williams, Hazim, Roni, Jiang, Mei, Esteve-Rudd, Julian, Diemer, Tanja, Lopes, Vanda S., Williams, David S. |
Abstract |
The retinal pigment epithelium (RPE) is a post-mitotic epithelial monolayer situated between the light-sensitive photoreceptors and the choriocapillaris. Given its vital functions for healthy vision, the RPE is a primary target for insults that result in blinding diseases, including age-related macular degeneration (AMD). One such function is the phagocytosis and digestion of shed photoreceptor outer segments. In the present study, we examined the process of trafficking of outer segment disk membranes in live cultures of primary mouse RPE, using high speed spinning disk confocal microscopy. This approach has enabled us to track phagosomes, and determine parameters of their motility, which are important for their efficient degradation. |
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