Chapter title |
Identification of Tyrosine O Sulfated Proteins in Cow Retina and the 661W Cell Line
|
---|---|
Chapter number | 86 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-17121-0_86 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
|
Authors |
Yogita Kanan, Muayyad R. Al-Ubaidi, Kanan, Yogita, Al-Ubaidi, Muayyad R. |
Abstract |
Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by 'Sulfinator' to determine potential sulfated tyrosines. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Librarian | 1 | 25% |
Student > Bachelor | 1 | 25% |
Lecturer > Senior Lecturer | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Neuroscience | 1 | 25% |
Medicine and Dentistry | 1 | 25% |
Unknown | 1 | 25% |