Chapter title |
Characterization of Antibodies to Identify Cellular Expression of Dopamine Receptor 4
|
---|---|
Chapter number | 88 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-17121-0_88 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
|
Authors |
Janise D. Deming, Kathleen Van Craenenbroeck, Yun Sung Eom, Eun-Jin Lee, Cheryl Mae Craft, Deming, Janise D., Van Craenenbroeck, Kathleen, Eom, Yun Sung, Lee, Eun-Jin, Craft, Cheryl Mae |
Abstract |
The dopamine receptor D4 (DRD4) plays an important role in vision. In order to study the DRD4 expression in vivo, it is important to have antibodies that are specific for DRD4 for both immunoblot and immunohistochemical (IHC) applications. In this study, six antibodies raised against DRD4 peptides were tested in vitro, using transfected mammalian cells, and in vivo, using mouse retinas. Three Santa Cruz (SC) antibodies, D-16, N-20, and R-20, were successful in IHC of transfected DRD4; however, N-20 was the only one effective on immunoblot analysis in DRD4 transfected cells and IHC of mouse retinal sections, while R-20, 2B9, and Antibody Verify AAS63631C were non-specific or below detection. |
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