Chapter title |
Cell Fate of Müller Cells During Photoreceptor Regeneration in an N -Methyl- N -nitrosourea-Induced Retinal Degeneration Model of Zebrafish
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Chapter number | 91 |
Book title |
Retinal Degenerative Diseases
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Published in |
Advances in experimental medicine and biology, January 2016
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DOI | 10.1007/978-3-319-17121-0_91 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
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Authors |
Kazuhiro Ogai, Suguru Hisano, Kayo Sugitani, Yoshiki Koriyama, Satoru Kato, Ogai, Kazuhiro, Hisano, Suguru, Sugitani, Kayo, Koriyama, Yoshiki, Kato, Satoru |
Abstract |
Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very recently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU(+) cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model. |
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