Chapter title |
Detection of CCN1 and CCN5 mRNA in Human Cancer Samples Using a Modified In Situ Hybridization Technique.
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Chapter number | 41 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6430-7_41 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
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Authors |
Priyanka Ghosh, Snigdha Banerjee, Gargi Maity, Archana De, Sushanta K. Banerjee Ph.D., Sushanta K. Banerjee |
Editors |
Masaharu Takigawa |
Abstract |
In situ hybridization is an ideal tool for the detection and localization of mRNA expression of specific gene(s) in tissue sections and cell lines for prognosis, predictive markers, and highlighted potential therapeutic targets. Given the importance of CCN1 and CCN5 in breast and pancreatic cancer progression, these two secretory proteins could be novel therapeutic targets. Thus, evaluating the distribution of mRNA of these targets using in situ hybridization could be important preclinical tools. This chapter describes a detailed in situ hybridization technique for the detection of CCN1 and CCN5 in formalin-fixed, paraffin-embedded patient samples of breast and pancreatic cancers. |
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