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Chapter title |
Western Blotting Analysis of CCN Proteins in Calcified Tissues.
|
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Chapter number | 5 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa D.D.S., Ph.D., Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no cross-reaction with other CCN member proteins. |