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Chapter title |
Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.
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Chapter number | 4 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6430-7_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
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Authors |
Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa D.D.S., Ph.D., Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 50% |
Other | 1 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 100% |