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Mendeley readers
Chapter title |
TGF-β1- and CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.
|
---|---|
Chapter number | 39 |
Book title |
CCN Proteins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_39 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Philip C. Trackman Ph.D., Debashree Saxena, Manish V. Bais, Philip C. Trackman |
Editors |
Masaharu Takigawa |
Abstract |
A simple method for the determination of relative levels of insoluble collagen accumulation in fibroblast cultures is presented. Confluent cell cultures are provided with sodium ascorbate which is then permissive for collagen deposition. At intervals, cultures are fixed and stained successively with sirius red and then crystal Violet to, respectively, assess for relative changes in collagen accumulation in response to factors such as TGF-β1 or matricellular CCN2 and changes in DNA content as an index of changes in cell density. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 4 | 20% |
Student > Master | 4 | 20% |
Student > Ph. D. Student | 3 | 15% |
Researcher | 2 | 10% |
Student > Bachelor | 1 | 5% |
Other | 3 | 15% |
Unknown | 3 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 45% |
Medicine and Dentistry | 3 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Chemistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 15% |