Chapter title |
Cell Biological Assays for Measuring Angiogenic Activities of CCN Proteins.
|
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Chapter number | 22 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Tsuyoshi Shimo D.D.S., Ph.D., Masaharu Takigawa D.D.S., Ph.D., Tsuyoshi Shimo, Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the action of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of connective tissue growth factor (CTGF/CCN2) and CCN2 neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 50% |
Student > Postgraduate | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 100% |