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Mendeley readers
Chapter title |
Production of Recombinant CCN Proteins by Brevibacillus choshinensis.
|
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Chapter number | 9 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6430-7_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Hiroshi Hanagata, Makoto Mizukami |
Editors |
Masaharu Takigawa |
Abstract |
Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to efficient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 33% |
Student > Master | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Nursing and Health Professions | 1 | 33% |
Unknown | 1 | 33% |