Chapter title |
Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two-Hybrid System.
|
---|---|
Chapter number | 15 |
Book title |
CCN Proteins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Mitsuhiro Hoshijima, Takako Hattori, Masaharu Takigawa D.D.S., Ph.D., Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Yeast two-hybrid screening is a powerful method to identify proteins that interact with a protein of interest. CCN2 consists of four domains, and identification of new proteins that bind to individual domains of CCN2 may reveal a variety of CCN2 functions. To identify CCN2-interactive proteins that regulate CCN2 activity, we carried out GAL4-based yeast two-hybrid screening with a cDNA library derived from a chondrocytic cell line, HCS-2/8, with CCN2 cDNA used as a bait. In this chapter, we describe our methods for screening for CCN2 binding partners by this system in detail. This protocol may be applied to other CCN proteins as well. |
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