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Chapter title |
Analysis of Transcytosis of CCN2 by Chondrocytes.
|
---|---|
Chapter number | 33 |
Book title |
CCN Proteins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_33 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Kazumi Kawata, Satoshi Kubota, Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Transcytosis is a mechanism for the transcellular transport of biomolecules. Analysis of transcytosis is frequently performed in cells with distinct polarity, such as brain endothelial cells. However, in cells without evident polarity, analysis of transcytosis has not been performed. Here, we describe a method for analyzing transcytosis of a CCN family protein through chondrocytic cells having no apparent polarity. |