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Mendeley readers
Chapter title |
Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.
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Chapter number | 29 |
Book title |
CCN Proteins
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6430-7_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
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Authors |
Azusa Maeda D.D.S., Ph.D., Marian Young Ph.D., Mitsuaki Ono D.D.S., Ph.D., Azusa Maeda, Marian Young, Mitsuaki Ono |
Editors |
Masaharu Takigawa |
Abstract |
Analysis of CCN4 function in bone was assessed using both gain and loss of function approaches. In mice this was done by genetic engineering and homologous recombination to create mice with complete ablation of the protein. For human skeletal cells adenoviral gene transfer and shRNA were used for gain and loss of function respectively. Here we describe procedures used to make and then analyze osteogenic and osteoclastic cells with or without CCN4 to determine its role in osteogenic differentiation. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 100% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 1 | 100% |