CCN Proteins

Table of Contents

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   Book Overview
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   Chapter 1 The CCN Proteins: An Overview.
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   Chapter 2 Gene Expression Analysis of CCN Proteins: Whole-Mount In Situ Hybridization of Ccn2 in Developing Calcified Tissues.
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   Chapter 3 Expression of CCN Genes and Proteins in Human Skin: Methods and Protocols.
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   Chapter 4 Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.
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   Chapter 5 Western Blotting Analysis of CCN Proteins in Calcified Tissues.
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   Chapter 6 Immunohistochemical Analysis of CCN Proteins in Calcified Tissues.
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   Chapter 7 Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis.
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   Chapter 8 Production of Recombinant CCN2 Protein in Escherichia coli.
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    Chapter 9 Production of Recombinant CCN Proteins by Brevibacillus choshinensis.
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    Chapter 10 Production of Recombinant CCN2 Protein by Mammalian Cells.
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    Chapter 11 In Vitro Transfection with and Expression of CCN Family of Genes.
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    Chapter 12 Preparation of Module-Specific Antibodies Against CCN Family Members.
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    Chapter 13 ELISA of CCN Family Proteins in Body Fluids Including Serum and Plasma.
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    Chapter 14 Analysis of Signaling Pathways Activated by CCN Proteins.
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    Chapter 15 Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two-Hybrid System.
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    Chapter 16 Protocols for Screening Peptide Motifs Binding to CCN Family Proteins.
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    Chapter 17 Evaluation of Molecular Interaction between CCN2 Protein and Its Binding Partners by Surface Plasmon Resonance (SPR).
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    Chapter 18 Promoter Analyses of CCN Genes.
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    Chapter 19 Analysis of Posttranscriptional Regulation of CCN Genes.
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    Chapter 20 Protein Imaging of CCN2 and CCN3 in Living Cells.
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    Chapter 21 Cell Biological Assays for Measuring Chondrogenic Activities of CCN2 Protein.
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    Chapter 22 Cell Biological Assays for Measuring Angiogenic Activities of CCN Proteins.
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    Chapter 23 Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins.
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    Chapter 24 Separation and Enrichment of Hematopoietic Stem Cells for CCN Studies.
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    Chapter 25 In Vivo Evaluation of Cartilage Regenerative Effects of CCN2 Protein.
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    Chapter 26 Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress.
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    Chapter 27 The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of Ccn3 Transgenic and Knockout Mice.
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    Chapter 28 Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing LacZ Reporters.
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    Chapter 29 Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.
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    Chapter 30 Construction and Analysis of an Allelic Series of Ccn1 Knockin Mice.
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    Chapter 31 Production and Analysis of Conditional KO Mice of CCN2 in Kidney.
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    Chapter 32 Generation and Analysis of Cartilage-Specific CCN2 Overexpression in Transgenic Mice.
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    Chapter 33 Analysis of Transcytosis of CCN2 by Chondrocytes.
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    Chapter 34 CCN2 in Skin Fibrosis.
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    Chapter 35 Studying the CCN Proteins in Fibrosis.
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    Chapter 36 Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases: Kidney Fibrosis.
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    Chapter 37 Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases: Liver Fibrosis.
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    Chapter 38 Cellular or Exosomal microRNAs Associated with CCN Gene Expression in Liver Fibrosis.
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    Chapter 39 TGF-β1- and CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.
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    Chapter 40 CCN Detection of Cancer Tissues by Immunohistochemistry Staining.
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    Chapter 41 Detection of CCN1 and CCN5 mRNA in Human Cancer Samples Using a Modified In Situ Hybridization Technique.
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    Chapter 42 Analysis of Pathological Activities of CCN Proteins in Bone Metastasis.
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    Chapter 43 Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy.
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    Chapter 44 Method for Analysis of Matrix Degradation by CCN2 Through the MMP/TIMP System.
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    Chapter 45 An Analysis of Pathological Activities of CCN Proteins in Joint Disorders: Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells.
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    Chapter 46 Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies.