Chapter title |
Immunohistochemical Analysis of CCN Proteins in Calcified Tissues.
|
---|---|
Chapter number | 6 |
Book title |
CCN Proteins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa D.D.S., Ph.D., Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Immunohistochemistry is a major technique to determine the distribution and localization of differentially produced proteins in the context of an intact tissue. It exploits one of the properties of antibodies, specific binding to an antigen, i.e., to the epitope of its target protein, in combination with a color-developing enzymatic reaction or tagged fluorophore. We have clarified the spatial and temporal expression patterns of CCN family proteins in several different types of animal tissues by using this immunohistochemical technique to support our corresponding data obtained in vitro. In this chapter, we provide our protocol for immunohistochemistry optimized for paraffin-embedded sections after having determined the optimal conditions for the use of antibodies against each member of the CCN family. |
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.