You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Production of Recombinant CCN2 Protein in Escherichia coli.
|
---|---|
Chapter number | 8 |
Book title |
CCN Proteins
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6430-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6428-4, 978-1-4939-6430-7
|
Authors |
Eriko Aoyama, Takako Hattori, Satoshi Kubota, Masaharu Takigawa |
Editors |
Masaharu Takigawa |
Abstract |
Recombinant proteins are important tools for understanding molecular functions in vitro. Recent progress in the generation of recombinant proteins is amazing. However, when we plan to produce them, we should choose the best method according to the nature and the use of the target recombinant protein. Degradation and mis-folding are major problems in producing active recombinant CCN2. The method shown in this chapter describes the appropriate conditions under which we can produce CCN2 and its truncated fragments in Escherichia coli. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 33% |
Student > Doctoral Student | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 67% |
Biochemistry, Genetics and Molecular Biology | 1 | 33% |