Chapter title |
Acidosis Promotes Metastasis Formation by Enhancing Tumor Cell Motility.
|
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Chapter number | 27 |
Book title |
Oxygen Transport to Tissue XXXVII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-1-4939-3023-4_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3022-7, 978-1-4939-3023-4
|
Authors |
Riemann, A, Schneider, B, Gündel, D, Stock, C, Gekle, M, Thews, O, A. Riemann, B. Schneider, D. Gündel, C. Stock, M. Gekle, O. Thews |
Editors |
Clare E. Elwell, Terence S. Leung, David K. Harrison |
Abstract |
The tumor microenvironment is characterized by hypoxia, acidosis as well as other metabolic and biochemical alterations. Its role in cancer progression is increasingly appreciated especially on invasive capacity and the formation of metastasis. The effect of acidosis on metastasis formation of two rat carcinoma cell lines was studied in the animal model. In order to analyze the pH dependency of different steps of metastasis formation, invasiveness, cell adhesion and migration of AT-1 prostate cancer cells as well as possible underlying cell signaling pathways were studied in vitro.Acidosis significantly increased the formation of lung metastases of both tumor cell lines in vivo. In vitro, extracellular acidosis neither enhanced invasiveness nor affected cell adhesion to a plastic or to an endothelial layer. However, cellular motility was markedly elevated at pH 6.6 and this effect was sustained even when extracellular pH was switched back to pH 7.4. When analyzing the underlying mechanism, a prominent role of ROS in the induction of migration was observed. Signaling through the MAP kinases ERK1/2 and p38 as well as Src family kinases was not involved. Thus, cancer cells in an acidic microenvironment can acquire enhanced motility, which is sustained even if the tumor cells leave their acidic microenvironment e.g. by entering the blood stream. This increase depended on elevated ROS production and may contribute to the augmented formation of metastases of acidosis-primed tumor cells in vivo. |
X Demographics
Geographical breakdown
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 31% |
Researcher | 4 | 15% |
Student > Postgraduate | 3 | 12% |
Student > Bachelor | 2 | 8% |
Unknown | 9 | 35% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Agricultural and Biological Sciences | 3 | 12% |
Medicine and Dentistry | 2 | 8% |
Nursing and Health Professions | 1 | 4% |
Chemical Engineering | 1 | 4% |
Other | 2 | 8% |
Unknown | 13 | 50% |