Chapter title |
Validation of a New Semi-Automated Technique to Evaluate Muscle Capillarization.
|
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Chapter number | 11 |
Book title |
Oxygen Transport to Tissue XXXVII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-1-4939-3023-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3022-7, 978-1-4939-3023-4
|
Authors |
Sam B. Ballak, Moi H. Yap, Peter J. Harding, Hans Degens |
Editors |
Clare E. Elwell, Terence S. Leung, David K. Harrison |
Abstract |
The method of capillary domains has often been used to study capillarization of skeletal and heart muscle. However, the conventional data processing method using a digitizing tablet is an arduous and time-consuming task. Here we compare a new semi-automated capillary domain data collection and analysis in muscle tissue with the standard capillary domain method. The capillary density (1481 ± 59 vs. 1447 ± 54 caps mm(-2); R(2):0.99; P < 0.01) and heterogeneity of capillary spacing (0.085 ± 0.002 vs. 0.085 ± 0.002; R(2):0.95; P < 0.01) were similar in both methods. The fiber cross-sectional area correlated well between the methods (R(2):0.84; P < 0.01) and did not differ significantly (~8 % larger in the old than new method at P = 0.08). The latter was likely due to differences in outlining the contours between the two methods. In conclusion, the semi-automated method gives quantitatively and qualitatively similar data as the conventional method and saves a considerable amount of time. |
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Demographic breakdown
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Researcher | 2 | 25% |
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Other | 1 | 13% |
Unknown | 1 | 13% |
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Unknown | 2 | 25% |