Chapter title |
Simultaneous Measurement of Dissolved Oxygen Pressure and Oxyhemoglobin Spectra in Solution.
|
---|---|
Chapter number | 48 |
Book title |
Oxygen Transport to Tissue XXXVII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-1-4939-3023-4_48 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3022-7, 978-1-4939-3023-4
|
Authors |
Maritoni Litorja, Jeeseong C. Hwang |
Editors |
Clare E. Elwell, Terence S. Leung, David K. Harrison |
Abstract |
The measurement of the spatial distribution of oxygen saturation (sO2) in superficial tissues using optical reflectance imaging has been useful in the clinical venue especially in temporally demanding applications such as monitoring tissue oxygenation during surgery. The measurement is based on relative spectrometry of oxy- and deoxyhemoglobin in tissues. We titrated deoxyhemoglobin with oxygen gas and simultaneously measured the dissolved oxygen pressure and the visible absorbance spectra to verify spectral shapes at different saturations. sO2 values derived from the measured pO2 are compared to those derived from the hemoglobin spectra at various stages of oxygenation. |
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