Chapter title |
Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
|
---|---|
Chapter number | 5 |
Book title |
Mitochondria
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6824-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6822-0, 978-1-4939-6824-4
|
Authors |
Bernadette Schreiner, Maria Ankarcrona |
Editors |
Dejana Mokranjac, Fabiana Perocchi |
Abstract |
During the last decades, increasing evidence indicated that subcellular organelles do not exist as autarkic units but instead communicate constantly and extensively with each other in various ways. Some communication, for example, the exchange of small molecules, requires the marked convergence of two distinct organelles for a certain period of time. The cross talk between endoplasmic reticulum (ER) and mitochondria, two subcellular organelles of utmost importance for cellular bioenergetics and protein homeostasis, has been increasingly investigated under the last years. This development was significantly driven by the establishment of optimized subcellular fractionation techniques. In this chapter, we will describe and critically discuss the currently used protocol for the isolation of the membrane fraction containing mitochondria-associated membranes (MAM). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 25% |
Student > Bachelor | 2 | 13% |
Researcher | 2 | 13% |
Unknown | 8 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 3 | 19% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 8 | 50% |