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Mitochondria

Overview of attention for book
Cover of 'Mitochondria'

Table of Contents

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    Book Overview
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    Chapter 1 A Guide to Computational Methods for Predicting Mitochondrial Localization
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    Chapter 2 Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
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    Chapter 3 Isolation of Mitochondria from Saccharomyces cerevisiae
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    Chapter 4 Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
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    Chapter 5 Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
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    Chapter 6 Label-Free Quantitative Analysis of Mitochondrial Proteomes Using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and “Total Protein Approach”
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    Chapter 7 Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
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    Chapter 8 Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
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    Chapter 9 Chemical Crosslinking in Intact Mitochondria
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    Chapter 10 Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
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    Chapter 11 Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
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    Chapter 12 Localizing mRNAs Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
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    Chapter 13 Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
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    Chapter 14 Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
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    Chapter 15 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
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    Chapter 16 New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
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    Chapter 17 Single Molecule Tracking and Localization of Mitochondrial Protein Complexes in Live Cells
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    Chapter 18 Analysis of Yeast Mitochondria by Electron Microscopy
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    Chapter 19 Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography
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    Chapter 20 Assays for Mitophagy in Yeast
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    Chapter 21 Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
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    Chapter 22 Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
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    Chapter 23 Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by qRT-PCR and RNAseq
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    Chapter 24 Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines
Attention for Chapter 13: Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
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Chapter title
Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
Chapter number 13
Book title
Mitochondria
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6824-4_13
Pubmed ID
28276021
Book ISBNs
978-1-4939-6822-0, 978-1-4939-6824-4
Authors

Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, Holger Prokisch

Editors

Dejana Mokranjac, Fabiana Perocchi

Abstract

Working with isolated mitochondria is the gold standard approach to investigate the function of the electron transport chain in tissues, free from the influence of other cellular factors. In this chapter, we outline a detailed protocol to measure the rate of oxygen consumption (OCR) with the high-throughput analyzer Seahorse XF96. More importantly, this protocol wants to provide practical tips for handling many different samples at once, and take a real advantage of using a high-throughput system. As a proof of concept, we have isolated mitochondria from brain, heart, liver, muscle, kidney, and lung of a wild-type mouse, and measured basal respiration (State II), ADP-stimulated respiration (State III), non-ADP-stimulated respiration (State IVo), and FCCP-stimulated respiration (State IIIu) using respiratory substrates specific to the respiratory chain complex I (RCCI) and complex II (RCCII). Mitochondrial purification and Seahorse runs were performed in less than eight working hours.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 105 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 105 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 17%
Researcher 15 14%
Student > Bachelor 15 14%
Professor > Associate Professor 5 5%
Other 4 4%
Other 12 11%
Unknown 36 34%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 30 29%
Agricultural and Biological Sciences 13 12%
Neuroscience 9 9%
Immunology and Microbiology 4 4%
Medicine and Dentistry 4 4%
Other 4 4%
Unknown 41 39%

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