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Mitochondria

Overview of attention for book
Cover of 'Mitochondria'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Guide to Computational Methods for Predicting Mitochondrial Localization
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    Chapter 2 Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
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    Chapter 3 Isolation of Mitochondria from Saccharomyces cerevisiae
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    Chapter 4 Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
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    Chapter 5 Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
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    Chapter 6 Label-Free Quantitative Analysis of Mitochondrial Proteomes Using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and “Total Protein Approach”
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    Chapter 7 Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
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    Chapter 8 Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
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    Chapter 9 Chemical Crosslinking in Intact Mitochondria
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    Chapter 10 Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
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    Chapter 11 Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
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    Chapter 12 Localizing mRNAs Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
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    Chapter 13 Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
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    Chapter 14 Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
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    Chapter 15 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
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    Chapter 16 New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
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    Chapter 17 Single Molecule Tracking and Localization of Mitochondrial Protein Complexes in Live Cells
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    Chapter 18 Analysis of Yeast Mitochondria by Electron Microscopy
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    Chapter 19 Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography
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    Chapter 20 Assays for Mitophagy in Yeast
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    Chapter 21 Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
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    Chapter 22 Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
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    Chapter 23 Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by qRT-PCR and RNAseq
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    Chapter 24 Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines
Attention for Chapter 16: New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
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Chapter title
New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
Chapter number 16
Book title
Mitochondria
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6824-4_16
Pubmed ID
28276024
Book ISBNs
978-1-4939-6822-0, 978-1-4939-6824-4
Authors

Saroj G. Regmi, Stéphane G. Rolland

Editors

Dejana Mokranjac, Fabiana Perocchi

Abstract

Mitochondria are highly dynamic organelles that constantly fuse and divide. This process is essential as several neurodegenerative diseases have been associated with defects in mitochondrial fusion or fission. Several tools have been developed over the years to visualize mitochondria in organisms such as Caenorhabditis elegans. Combining these tools with the powerful genetics of C. elegans has led to the discovery of new regulators of mitochondrial morphology. In this chapter, we present additional tools to further characterize mitochondrial morphology as well as regulators of mitochondrial morphology. Specifically, we introduce a photoactivatable mitoGFP (PAmitoGFP) that allows to investigate the connectivity of complex mitochondrial networks. In addition, we describe an immunostaining protocol that enables localization studies of these newly identified regulators of mitochondrial morphology.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Student > Bachelor 2 18%
Lecturer 1 9%
Student > Doctoral Student 1 9%
Professor 1 9%
Other 1 9%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Agricultural and Biological Sciences 2 18%
Psychology 1 9%
Neuroscience 1 9%
Unknown 3 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2018.
All research outputs
#20,413,129
of 22,963,381 outputs
Outputs from Methods in molecular biology
#9,917
of 13,136 outputs
Outputs of similar age
#268,229
of 307,884 outputs
Outputs of similar age from Methods in molecular biology
#221
of 281 outputs
Altmetric has tracked 22,963,381 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,136 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,884 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 281 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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