Chapter title |
Chemical Crosslinking in Intact Mitochondria
|
---|---|
Chapter number | 9 |
Book title |
Mitochondria
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6824-4_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6822-0, 978-1-4939-6824-4
|
Authors |
Rupa Banerjee, Umut Günsel, Dejana Mokranjac |
Editors |
Dejana Mokranjac, Fabiana Perocchi |
Abstract |
Many mitochondrial proteins perform their functions as components of large, multimeric complexes. Chemical crosslinking is a powerful method to analyze protein-protein interactions within such complexes. Using membrane-permeable crosslinkers and isolated intact mitochondria, protein-protein interactions that are secluded by two mitochondrial membranes can be readily analyzed in physiologically active, isolated organelles under a variety of physiological and pathophysiological conditions. Here, we describe two methods for chemical crosslinking in intact yeast mitochondria. The first method enables the analysis of ATP-dependent remodeling of mitochondrial protein complexes while the second one allows the identification of crosslinking partners of a protein of interest. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 38% |
Student > Postgraduate | 2 | 25% |
Researcher | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Agricultural and Biological Sciences | 2 | 25% |
Unknown | 3 | 38% |