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Mitochondria

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Cover of 'Mitochondria'

Table of Contents

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    Book Overview
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    Chapter 1 A Guide to Computational Methods for Predicting Mitochondrial Localization
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    Chapter 2 Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
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    Chapter 3 Isolation of Mitochondria from Saccharomyces cerevisiae
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    Chapter 4 Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
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    Chapter 5 Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
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    Chapter 6 Label-Free Quantitative Analysis of Mitochondrial Proteomes Using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and “Total Protein Approach”
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    Chapter 7 Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
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    Chapter 8 Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
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    Chapter 9 Chemical Crosslinking in Intact Mitochondria
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    Chapter 10 Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
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    Chapter 11 Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
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    Chapter 12 Localizing mRNAs Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
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    Chapter 13 Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
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    Chapter 14 Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
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    Chapter 15 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
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    Chapter 16 New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
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    Chapter 17 Single Molecule Tracking and Localization of Mitochondrial Protein Complexes in Live Cells
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    Chapter 18 Analysis of Yeast Mitochondria by Electron Microscopy
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    Chapter 19 Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography
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    Chapter 20 Assays for Mitophagy in Yeast
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    Chapter 21 Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
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    Chapter 22 Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
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    Chapter 23 Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by qRT-PCR and RNAseq
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    Chapter 24 Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines
Attention for Chapter 14: Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
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Chapter title
Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
Chapter number 14
Book title
Mitochondria
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6824-4_14
Pubmed ID
28276022
Book ISBNs
978-1-4939-6822-0, 978-1-4939-6824-4
Authors

Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, Hiromi Imamura

Editors

Dejana Mokranjac, Fabiana Perocchi

Abstract

Genetically encoded biosensors utilizing the Förster resonance energy transfer (FRET) are powerful tools for live cell imaging of various cellular processes. Our group has previously developed a series of FRET-based biosensors, named "ATeam," for visualization of ATP levels inside a single living cell. ATeam not only provides a window of insight into a single cell but also allows for visualization of ATP levels in mitochondrial matrix of a single living cell. This novel tool is able to monitor alterations in cellular ATP in response to various treatments in real time. Here we present a method for the evaluation of ATP levels in mitochondria in living cells by using ATeam. At the end of this chapter, an example of experimental results is described for a better understanding of the presented procedure.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 59 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 24%
Student > Bachelor 9 15%
Student > Master 7 12%
Researcher 6 10%
Professor > Associate Professor 4 7%
Other 9 15%
Unknown 10 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 39%
Agricultural and Biological Sciences 7 12%
Physics and Astronomy 3 5%
Neuroscience 3 5%
Immunology and Microbiology 2 3%
Other 4 7%
Unknown 17 29%

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