You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
|
|---|---|
| Chapter number | 15 |
| Book title |
Mitochondria
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6824-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6822-0, 978-1-4939-6824-4
|
| Authors |
María Teresa Alonso, Paloma Navas-Navarro, Javier García-Sancho |
| Editors |
Dejana Mokranjac, Fabiana Perocchi |
| Abstract |
Mitochondrial Ca(2+) homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca(2+). Ca(2+) concentration in mitochondria ([Ca(2+)]M) is low at rest (about 10(-7) M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca(2+) uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca(2+) transport. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 60% |
| Student > Doctoral Student | 1 | 20% |
| Student > Master | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 40% |
| Neuroscience | 2 | 40% |
| Unknown | 1 | 20% |