↓ Skip to main content

Mitochondria

Overview of attention for book
Cover of 'Mitochondria'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 A Guide to Computational Methods for Predicting Mitochondrial Localization
  3. Altmetric Badge
    Chapter 2 Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
  4. Altmetric Badge
    Chapter 3 Isolation of Mitochondria from Saccharomyces cerevisiae
  5. Altmetric Badge
    Chapter 4 Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
  6. Altmetric Badge
    Chapter 5 Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
  7. Altmetric Badge
    Chapter 6 Label-Free Quantitative Analysis of Mitochondrial Proteomes Using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and “Total Protein Approach”
  8. Altmetric Badge
    Chapter 7 Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
  9. Altmetric Badge
    Chapter 8 Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
  10. Altmetric Badge
    Chapter 9 Chemical Crosslinking in Intact Mitochondria
  11. Altmetric Badge
    Chapter 10 Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
  12. Altmetric Badge
    Chapter 11 Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
  13. Altmetric Badge
    Chapter 12 Localizing mRNAs Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
  14. Altmetric Badge
    Chapter 13 Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
  15. Altmetric Badge
    Chapter 14 Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
  16. Altmetric Badge
    Chapter 15 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
  17. Altmetric Badge
    Chapter 16 New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
  18. Altmetric Badge
    Chapter 17 Single Molecule Tracking and Localization of Mitochondrial Protein Complexes in Live Cells
  19. Altmetric Badge
    Chapter 18 Analysis of Yeast Mitochondria by Electron Microscopy
  20. Altmetric Badge
    Chapter 19 Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography
  21. Altmetric Badge
    Chapter 20 Assays for Mitophagy in Yeast
  22. Altmetric Badge
    Chapter 21 Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
  23. Altmetric Badge
    Chapter 22 Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
  24. Altmetric Badge
    Chapter 23 Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by qRT-PCR and RNAseq
  25. Altmetric Badge
    Chapter 24 Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines
Attention for Chapter 21: Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
8 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
Chapter number 21
Book title
Mitochondria
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6824-4_21
Pubmed ID
Book ISBNs
978-1-4939-6822-0, 978-1-4939-6824-4
Authors

Konstantinos Palikaras, Nektarios Tavernarakis

Editors

Dejana Mokranjac, Fabiana Perocchi

Abstract

Eukaryotic cells heavily depend on ATP generated by oxidative phosphorylation (OXPHOS) within mitochondria. Besides being the main suppliers of cell's energy, mitochondria also provide an additional compartment for a wide range of cellular processes and metabolic pathways. Mitochondria constantly undergo fusion/fission events and form a mitochondrial network, which is a highly dynamic, tubular structure allowing for rapid and continuous exchange of genetic material, as well as, targeting dysfunctional mitochondria for degradation through mitochondrial selective autophagy (mitophagy). Mitophagy mediates the elimination of damaged and/or superfluous organelles, maintaining mitochondrial and cellular homeostasis. In this chapter, we present two versatile, noninvasive methods, developed for monitoring in vivo mitophagy in C. elegans. These procedures enable the assessment of mitophagy in several cell types during development or under stress conditions. Investigating the role of mitophagy at the organismal level is essential for the development of therapeutic interventions against age-related diseases.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 38%
Professor 2 25%
Researcher 1 13%
Student > Ph. D. Student 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 1 13%
Neuroscience 1 13%
Unknown 1 13%