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Mitochondria

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Cover of 'Mitochondria'

Table of Contents

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    Book Overview
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    Chapter 1 A Guide to Computational Methods for Predicting Mitochondrial Localization
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    Chapter 2 Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues
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    Chapter 3 Isolation of Mitochondria from Saccharomyces cerevisiae
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    Chapter 4 Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes
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    Chapter 5 Isolation of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue
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    Chapter 6 Label-Free Quantitative Analysis of Mitochondrial Proteomes Using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and “Total Protein Approach”
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    Chapter 7 Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
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    Chapter 8 Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells
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    Chapter 9 Chemical Crosslinking in Intact Mitochondria
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    Chapter 10 Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression
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    Chapter 11 Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast
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    Chapter 12 Localizing mRNAs Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation
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    Chapter 13 Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues Using Seahorse XF96 Analyzer
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    Chapter 14 Application of FRET-Based Biosensor “ATeam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells
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    Chapter 15 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake
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    Chapter 16 New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis elegans
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    Chapter 17 Single Molecule Tracking and Localization of Mitochondrial Protein Complexes in Live Cells
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    Chapter 18 Analysis of Yeast Mitochondria by Electron Microscopy
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    Chapter 19 Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography
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    Chapter 20 Assays for Mitophagy in Yeast
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    Chapter 21 Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans
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    Chapter 22 Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells
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    Chapter 23 Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by qRT-PCR and RNAseq
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    Chapter 24 Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines
Attention for Chapter 7: Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
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Chapter title
Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry
Chapter number 7
Book title
Mitochondria
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6824-4_7
Pubmed ID
Book ISBNs
978-1-4939-6822-0, 978-1-4939-6824-4
Authors

Takashi Tatsuta

Editors

Dejana Mokranjac, Fabiana Perocchi

Abstract

Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 38%
Professor > Associate Professor 2 25%
Student > Doctoral Student 1 13%
Researcher 1 13%
Student > Master 1 13%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Arts and Humanities 1 13%
Nursing and Health Professions 1 13%
Agricultural and Biological Sciences 1 13%
Computer Science 1 13%
Other 0 0%
Unknown 1 13%