Chapter title |
Analysis of Yeast Mitochondria by Electron Microscopy
|
---|---|
Chapter number | 18 |
Book title |
Mitochondria
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6824-4_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6822-0, 978-1-4939-6824-4
|
Authors |
Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, Benedikt Westermann |
Editors |
Dejana Mokranjac, Fabiana Perocchi |
Abstract |
Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method. |
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Demographic breakdown
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Student > Master | 5 | 19% |
Researcher | 4 | 15% |
Professor | 2 | 7% |
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Other | 2 | 7% |
Unknown | 6 | 22% |
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Physics and Astronomy | 1 | 4% |
Other | 3 | 11% |
Unknown | 7 | 26% |