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Chapter title |
Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels
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Chapter number | 25 |
Book title |
Zebrafish
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3771-4_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
Authors |
Eric J. Horstick, Kathryn M. Tabor, Diana C. Jordan, Harold A. Burgess |
Abstract |
Advances in genetic technologies enable the highly selective expression of transgenes in targeted neuronal cell types. Transgene expression can be used to noninvasively ablate, silence or activate neurons, providing a tool to probe their contribution to the control of behavior or physiology. Here, we describe the use of the tetrodotoxin (TTX)-resistant voltage-gated sodium channel Nav1.5 for either sensitizing neurons to depolarizing input, or isolating targeted neurons from surrounding neural activity, and methods for selective neuronal ablation using the bacterial nitroreductase NfsB. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 33% |
Researcher | 2 | 17% |
Lecturer | 1 | 8% |
Student > Master | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 4 | 33% |
Agricultural and Biological Sciences | 3 | 25% |
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Unknown | 3 | 25% |