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Zebrafish

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Cover of 'Zebrafish'

Table of Contents

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    Book Overview
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    Chapter 1 Chemical Screening in Zebrafish
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    Chapter 2 TALEN-Mediated Mutagenesis and Genome Editing
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    Chapter 3 Zebrafish
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    Chapter 4 Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System
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    Chapter 5 Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish
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    Chapter 6 Testing of Cis -Regulatory Elements by Targeted Transgene Integration in Zebrafish Using PhiC31 Integrase
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    Chapter 7 Zebrafish
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    Chapter 8 Construction of the Inbred Strain
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    Chapter 9 Zebrafish as a Model for the Study of Solid Malignancies
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    Chapter 10 Melanoma Regression and Recurrence in Zebrafish
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    Chapter 11 Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model
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    Chapter 12 Modeling Leukemogenesis in the Zebrafish Using Genetic and Xenograft Models
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    Chapter 13 Zebrafish
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    Chapter 14 Zebrafish
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    Chapter 15 Zebrafish
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    Chapter 16 Studying Lipid Metabolism and Transport During Zebrafish Development
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    Chapter 17 Targeted Electroporation in Embryonic, Larval, and Adult Zebrafish
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    Chapter 18 Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy
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    Chapter 19 Zebrafish
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    Chapter 20 Quantifying Aggressive Behavior in Zebrafish
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    Chapter 21 Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
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    Chapter 22 A Practical Guide to Light Sheet Microscopy
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    Chapter 23 Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish
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    Chapter 24 Fiber Optic-Based Photostimulation of Larval Zebrafish
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    Chapter 25 Genetic Ablation, Sensitization, and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels
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    Chapter 26 Erratum
Attention for Chapter 6: Testing of Cis -Regulatory Elements by Targeted Transgene Integration in Zebrafish Using PhiC31 Integrase
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Chapter title
Testing of Cis -Regulatory Elements by Targeted Transgene Integration in Zebrafish Using PhiC31 Integrase
Chapter number 6
Book title
Zebrafish
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3771-4_6
Pubmed ID
Book ISBNs
978-1-4939-3769-1, 978-1-4939-3771-4
Authors

Yavor Hadzhiev, Irene Miguel-Escalada, Darius Balciunas, Ferenc Müller

Abstract

Herein we present several strategies for testing the function of cis-regulatory elements using the PhiC31 integrase system. Firstly, we present two different strategies to analyze the activity of candidate enhancer elements. Targeted integration of candidate enhancers into the same genomic location circumvents the variability-associated random integration and position effects. This method is suitable for testing of candidate enhancers identified through computational or other analyses a priori. Secondly, we present methodology for targeted integration of BACs into the same genomic location(s). By using additional reporters integrated into a BAC, this enables experimental testing whether cis-regulatory elements are functional in the sequence inserted in the BAC.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 42%
Professor > Associate Professor 2 17%
Student > Ph. D. Student 2 17%
Professor 1 8%
Student > Bachelor 1 8%
Other 0 0%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 42%
Biochemistry, Genetics and Molecular Biology 4 33%
Psychology 1 8%
Medicine and Dentistry 1 8%
Unknown 1 8%