Chapter title |
Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model
|
---|---|
Chapter number | 11 |
Book title |
Zebrafish
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3771-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
Authors |
Claudia Tulotta, Shuning He, Lanpeng Chen, Arwin Groenewoud, Wietske van der Ent, Annemarie H. Meijer, Herman P. Spaink, B. Ewa Snaar-Jagalska, Tulotta, Claudia, He, Shuning, Chen, Lanpeng, Groenewoud, Arwin, Ent, Wietske van der, Meijer, Annemarie H., Spaink, Herman P., Snaar-Jagalska, B. Ewa |
Abstract |
The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible assay can be used as an intermediate step between in vitro techniques and the expensive, and time consuming, murine models of cancer invasion and metastasis.In this chapter, a detailed protocol to inject human cancer cells into the blood circulation of a zebrafish embryo is described; the engraftment procedure is then followed by visualization and quantification methods of tumor cell proliferation, invasion, and micrometastasis formation during subsequent larval development. Interaction with the host microenvironment is also considered. |
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