| Chapter title |
Zebrafish
|
|---|---|
| Chapter number | 3 |
| Book title |
Zebrafish
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3771-4_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
| Authors |
Auer, Thomas O, Del Bene, Filippo, Thomas O. Auer, Filippo Del Bene, Auer, Thomas O., Bene, Filippo Del |
| Abstract |
Targeting nucleases like zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) system have revolutionized genome-editing possibilities in many model organisms. They allow the generation of loss-of-function alleles by the introduction of double-strand breaks at defined sites within genes, but also more sophisticated genome-editing approaches have become possible. These include the integration of donor plasmid DNA into the genome by homology-independent repair mechanisms after CRISPR/Cas9-mediated cleavage. Here we present a protocol outlining the most important steps to target a genomic site and to integrate a donor plasmid at this defined locus. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 36% |
| Other | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Professor | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 6 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 44% |
| Agricultural and Biological Sciences | 3 | 12% |
| Medicine and Dentistry | 2 | 8% |
| Psychology | 1 | 4% |
| Neuroscience | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 24% |