Zebrafish
Humana Press
Chapter title |
Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
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Chapter number | 21 |
Book title |
Zebrafish
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3771-4_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
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Authors |
Michael B. Orger, Ruben Portugues, Orger, Michael B., Portugues, Ruben |
Abstract |
We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data. |
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