Zebrafish
Humana Press
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Timeline
Mendeley readers
| Chapter title |
TALEN-Mediated Mutagenesis and Genome Editing
|
|---|---|
| Chapter number | 2 |
| Book title |
Zebrafish
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3771-4_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
| Authors |
Alvin C. H. Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker, Ma, Alvin C. H., Chen, Yi, Blackburn, Patrick R., Ekker, Stephen C. |
| Abstract |
Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 19% |
| Student > Ph. D. Student | 5 | 16% |
| Student > Bachelor | 3 | 9% |
| Student > Doctoral Student | 3 | 9% |
| Professor > Associate Professor | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 10 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 34% |
| Agricultural and Biological Sciences | 3 | 9% |
| Neuroscience | 3 | 9% |
| Immunology and Microbiology | 2 | 6% |
| Psychology | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 9 | 28% |