Chapter title |
Zebrafish
|
---|---|
Chapter number | 7 |
Book title |
Zebrafish
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3771-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3769-1, 978-1-4939-3771-4
|
Authors |
Tanabe, Hideyuki, Seki, Masahide, Itoh, Mari, Deepak, Ailani, Lal, Pradeep, Horiuchi, Terumi, Suzuki, Yutaka, Kawakami, Koichi, Hideyuki Tanabe, Masahide Seki, Mari Itoh, Ailani Deepak, Pradeep Lal, Terumi Horiuchi, Yutaka Suzuki, Koichi Kawakami |
Abstract |
Gene expression profiling is a useful approach for deeper understanding of the specificity of cells, tissues, and organs in the transcriptional level. Recent development of high-throughput next-generation sequence (NGS) allows the RNA-seq method for this profiling. This method provides precise information of transcripts about the quantitation and the structure such as the splicing variants. In this chapter, we describe a method for gene expression profiling of GFP-positive cells from transgenic zebrafish by RNA-seq. We labeled specific cells in the brain with GFP by crossing a Gal4 driver line with the UAS:GFP line, isolated those cells by fluorescence-activated cell sorting (FACS), and analyzed by RNA-seq. |
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