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RNA Methylation

Overview of attention for book
Cover of 'RNA Methylation'

Table of Contents

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    Book Overview
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    Chapter 1 LC-MS Analysis of Methylated RNA
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    Chapter 2 Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry
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    Chapter 3 Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
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    Chapter 4 Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)
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    Chapter 5 Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
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    Chapter 6 Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
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    Chapter 7 Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)
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    Chapter 8 Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
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    Chapter 9 Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
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    Chapter 10 Analysis of High-Throughput RNA Bisulfite Sequencing Data
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    Chapter 11 Statistical Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite Sequencing
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    Chapter 12 High-Throughput Mapping of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
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    Chapter 13 RiboMeth-seq: Profiling of 2′-O-Me in RNA
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    Chapter 14 In Silico Identification of RNA Modifications from High-Throughput Sequencing Data Using HAMR
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    Chapter 15 High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)
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    Chapter 16 Transcriptome-Wide Mapping of N 1-Methyladenosine Methylome
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    Chapter 17 In Vitro Assays for RNA Methyltransferase Activity
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    Chapter 18 Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
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    Chapter 19 Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
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    Chapter 20 Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA
Attention for Chapter 6: Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
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Chapter title
Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
Chapter number 6
Book title
RNA Methylation
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6807-7_6
Pubmed ID
Book ISBNs
978-1-4939-6805-3, 978-1-4939-6807-7
Authors

Zsuzsanna Bodi, Rupert G. Fray, Bodi, Zsuzsanna, Fray, Rupert G.

Editors

Alexandra Lusser

Abstract

The base-modified nucleotide, N (6)-methyladenosine, is a relatively abundant modification found in the mRNA of most higher eukaryotes. Methylation levels can change dependent upon environmental conditions, cell differentiation state, or following knockdown of members of the methylase complex, and it is often useful to directly measure and compare N (6)-methyladenosine levels between samples. Two dimensional chromatography of radiolabeled nucleotides, following specific nuclease treatments, provides a robust, sensitive, and reproducible assay for this modification.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 29%
Researcher 5 24%
Student > Doctoral Student 1 5%
Unspecified 1 5%
Student > Master 1 5%
Other 1 5%
Unknown 6 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 38%
Agricultural and Biological Sciences 3 14%
Nursing and Health Professions 2 10%
Computer Science 1 5%
Unspecified 1 5%
Other 0 0%
Unknown 6 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2018.
All research outputs
#21,285,712
of 26,017,215 outputs
Outputs from Methods in molecular biology
#9,241
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Outputs of similar age
#253,622
of 326,891 outputs
Outputs of similar age from Methods in molecular biology
#180
of 295 outputs
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