↓ Skip to main content

RNA Methylation

Overview of attention for book
Cover of 'RNA Methylation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 LC-MS Analysis of Methylated RNA
  3. Altmetric Badge
    Chapter 2 Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry
  4. Altmetric Badge
    Chapter 3 Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
  5. Altmetric Badge
    Chapter 4 Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)
  6. Altmetric Badge
    Chapter 5 Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
  7. Altmetric Badge
    Chapter 6 Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
  8. Altmetric Badge
    Chapter 7 Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)
  9. Altmetric Badge
    Chapter 8 Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
  10. Altmetric Badge
    Chapter 9 Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
  11. Altmetric Badge
    Chapter 10 Analysis of High-Throughput RNA Bisulfite Sequencing Data
  12. Altmetric Badge
    Chapter 11 Statistical Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite Sequencing
  13. Altmetric Badge
    Chapter 12 High-Throughput Mapping of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
  14. Altmetric Badge
    Chapter 13 RiboMeth-seq: Profiling of 2′-O-Me in RNA
  15. Altmetric Badge
    Chapter 14 In Silico Identification of RNA Modifications from High-Throughput Sequencing Data Using HAMR
  16. Altmetric Badge
    Chapter 15 High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)
  17. Altmetric Badge
    Chapter 16 Transcriptome-Wide Mapping of N 1-Methyladenosine Methylome
  18. Altmetric Badge
    Chapter 17 In Vitro Assays for RNA Methyltransferase Activity
  19. Altmetric Badge
    Chapter 18 Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
  20. Altmetric Badge
    Chapter 19 Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
  21. Altmetric Badge
    Chapter 20 Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA
Attention for Chapter 9: Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 X user

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
9 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
Chapter number 9
Book title
RNA Methylation
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6807-7_9
Pubmed ID
Book ISBNs
978-1-4939-6805-3, 978-1-4939-6807-7
Authors

Amort, Thomas, Sun, Xueguang, Khokhlova-Cubberley, Daria, Lusser, Alexandra, Thomas Amort, Xueguang Sun, Daria Khokhlova-Cubberley, Alexandra Lusser

Editors

Alexandra Lusser

Abstract

While low-throughput RNA bisulfite sequencing is the method of choice to assess the methylation status of specific cytosines in candidate RNAs, the combination of bisulfite treatment of RNA with today's high-throughput sequencing techniques opens the door to methylation studies at nucleotide resolution on a transcriptome-wide scale. Below we describe a protocol for the transcriptome-wide analysis of total or fractionated poly(A)RNA in cells and tissues. Although the nature of the bisulfite sequencing protocol makes it comparably easy to translate from a low to a high-throughput approach, several critical points require attention before starting such a project. We describe a step-by-step protocol for planning and performing the experiment and analyzing the data.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 44%
Other 1 11%
Student > Postgraduate 1 11%
Student > Master 1 11%
Unknown 2 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Agricultural and Biological Sciences 2 22%
Nursing and Health Professions 1 11%
Unknown 2 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2017.
All research outputs
#15,452,475
of 22,962,258 outputs
Outputs from Methods in molecular biology
#5,372
of 13,136 outputs
Outputs of similar age
#193,771
of 308,511 outputs
Outputs of similar age from Methods in molecular biology
#109
of 303 outputs
Altmetric has tracked 22,962,258 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,136 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,511 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 303 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.