Thüring, Kathrin, Schmid, Katharina, Keller, Patrick, Helm, Mark, Kathrin Thüring, Katharina Schmid, Patrick Keller, Mark Helm
Editors
Alexandra Lusser
Abstract
The detection and quantification of methylated RNA can be beneficial to understand certain cellular regulation processes such as transcriptional modulation of gene expression, immune response, or epigenetic alterations. Therefore, it is necessary to have methods available, which are extremely sensitive and accurate, for instance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Here, we describe the preparation of RNA samples by enzymatic hydrolysis and the subsequent analysis of ribonucleosides by LC-MS/MS via NLS (Neutral loss scan) and DMRM (Dynamic multiple reaction monitoring). Also, we provide variations of these methods including chromatographic techniques and different kinds of quantification.
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2017.
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#17,345,186
of 26,017,215 outputs
Outputs from Methods in molecular biology
#5,540
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Outputs of similar age
#200,264
of 326,891 outputs
Outputs of similar age from Methods in molecular biology
#96
of 295 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. This one is in the 31st percentile – i.e., 31% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,425 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 57% of its peers.
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We're also able to compare this research output to 295 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.
