↓ Skip to main content

RNA Methylation

Overview of attention for book
Cover of 'RNA Methylation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 LC-MS Analysis of Methylated RNA
  3. Altmetric Badge
    Chapter 2 Comparative Analysis of Ribonucleic Acid Digests (CARD) by Mass Spectrometry
  4. Altmetric Badge
    Chapter 3 Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation
  5. Altmetric Badge
    Chapter 4 Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-Seq)
  6. Altmetric Badge
    Chapter 5 Mapping m6A at Individual-Nucleotide Resolution Using Crosslinking and Immunoprecipitation (miCLIP)
  7. Altmetric Badge
    Chapter 6 Detection and Quantification of N 6-Methyladenosine in Messenger RNA by TLC
  8. Altmetric Badge
    Chapter 7 Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)
  9. Altmetric Badge
    Chapter 8 Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing
  10. Altmetric Badge
    Chapter 9 Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing
  11. Altmetric Badge
    Chapter 10 Analysis of High-Throughput RNA Bisulfite Sequencing Data
  12. Altmetric Badge
    Chapter 11 Statistical Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite Sequencing
  13. Altmetric Badge
    Chapter 12 High-Throughput Mapping of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
  14. Altmetric Badge
    Chapter 13 RiboMeth-seq: Profiling of 2′-O-Me in RNA
  15. Altmetric Badge
    Chapter 14 In Silico Identification of RNA Modifications from High-Throughput Sequencing Data Using HAMR
  16. Altmetric Badge
    Chapter 15 High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)
  17. Altmetric Badge
    Chapter 16 Transcriptome-Wide Mapping of N 1-Methyladenosine Methylome
  18. Altmetric Badge
    Chapter 17 In Vitro Assays for RNA Methyltransferase Activity
  19. Altmetric Badge
    Chapter 18 Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo
  20. Altmetric Badge
    Chapter 19 Methylated mRNA Nucleotides as Regulators for Ribosomal Translation
  21. Altmetric Badge
    Chapter 20 Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA
Attention for Chapter 17: In Vitro Assays for RNA Methyltransferase Activity
Altmetric Badge

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
18 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
In Vitro Assays for RNA Methyltransferase Activity
Chapter number 17
Book title
RNA Methylation
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6807-7_17
Pubmed ID
Book ISBNs
978-1-4939-6805-3, 978-1-4939-6807-7
Authors

Sara Haag, Katherine E. Sloan, Claudia Höbartner, Markus T. Bohnsack

Editors

Alexandra Lusser

Abstract

RNA methyltransferases (MTases) are responsible for co- and posttranscriptional methylation of nucleotides in a wide variety of RNA substrates. Examination of the target specificity, catalytic activity, and function of these enzymes requires in vitro methylation assays. Here, we provide a detailed protocol for the methylation of in vitro transcripts, synthetic RNAs, and total cellular RNA using recombinant RNA methyltransferases and S-adenosylmethionine (SAM) as a methyl group donor. We describe how this method can be coupled to fluorographic detection of RNA methylation if (3)H-labeled SAM is used, and discuss alternative chromatography-based methods for the detection of methylated nucleotides, focusing on reversed-phase high-performance liquid chromatography (RP-HPLC). In both cases, mutagenesis of the methyltransferase or the RNA substrate can be employed to elucidate the catalytic mechanisms and target specificity of the enzymes. Together these approaches provide valuable insight into the action of RNA methyltransferases on the molecular level and serve as a basis for further functional characterization of RNA methyltransferases in vivo.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 33%
Student > Ph. D. Student 4 22%
Student > Postgraduate 2 11%
Student > Bachelor 2 11%
Student > Doctoral Student 1 6%
Other 2 11%
Unknown 1 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 50%
Chemistry 2 11%
Agricultural and Biological Sciences 1 6%
Nursing and Health Professions 1 6%
Unknown 5 28%