N (1)-Methyladenosine (m(1)A) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of m(1)A in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of m(1)A will be important for further functional investigations. Here, we describe a technique called "m(1)A-ID-Seq", which is based on m(1)A immunoprecipitation and the inherent ability of m(1)A to stall reverse transcription, to map m(1)A in the transcriptome. Utilizing this technique, highly confident m(1)A peaks can be obtained on a transcriptome-wide level.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2017.
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#15,452,475
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#5,372
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#109
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