| Chapter title |
Illustrating the Epitranscriptome at Nucleotide Resolution Using Methylation-iCLIP (miCLIP)
|
|---|---|
| Chapter number | 7 |
| Book title |
RNA Methylation
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6807-7_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6805-3, 978-1-4939-6807-7
|
| Authors |
George, Harry, Ule, Jernej, Hussain, Shobbir, Harry George, Jernej Ule, Shobbir Hussain, George, H, Ule, J, Hussain, S |
| Editors |
Alexandra Lusser |
| Abstract |
Next-generation sequencing technologies have enabled the transcriptome to be profiled at a previously unprecedented speed and depth. This yielded insights into fundamental transcriptomic processes such as gene transcription, RNA processing, and mRNA splicing. Immunoprecipitation-based transcriptomic methods such as individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) have also allowed high-resolution analysis of the RNA interactions of a protein of interest, thus revealing new regulatory mechanisms. We and others have recently modified this method to profile RNA methylation, and we refer to this customized technique as methylation-iCLIP (miCLIP). Variants of miCLIP have been used to map the methyl-5-cytosine (m5C) or methyl-6-adenosine (m6A) modification at nucleotide resolution in the human transcriptome. Here we describe the m5C-miCLIP protocol, discuss how it yields the nucleotide-resolution RNA modification maps, and comment on how these have contributed to the new field of molecular genetics research coined "epitranscriptomics." |
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Mendeley readers
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|---|---|---|
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| Researcher | 7 | 18% |
| Student > Bachelor | 6 | 16% |
| Student > Postgraduate | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 6 | 16% |
| Unknown | 7 | 18% |
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| Business, Management and Accounting | 1 | 3% |
| Other | 4 | 11% |
| Unknown | 7 | 18% |