RNA Methylation
Springer New York
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Timeline
Mendeley readers
| Chapter title |
High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-Seq)
|
|---|---|
| Chapter number | 15 |
| Book title |
RNA Methylation
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6807-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6805-3, 978-1-4939-6807-7
|
| Authors |
Eva Hrabeta-Robinson, Erin Marcus, Aaron E. Cozen, Eric M. Phizicky, Todd M. Lowe |
| Editors |
Alexandra Lusser |
| Abstract |
N (1)-methyladenosine (m(1)A), N (3)-methylcytidine (m(3)C), and N (1)-methylguanosine (m(1)G) are common in transfer RNA (tRNA) and tRNA-derived fragments. These modifications alter Watson-Crick base-pairing, and cause pauses or stops during reverse transcription required for most high-throughput RNA sequencing protocols, resulting in inefficient detection of methyl-modified RNAs. Here, we describe a procedure to demethylate RNAs containing m(1)A, m(3)C, or m(1)G using the Escherichia coli dealkylating enzyme AlkB, along with instructions for subsequent processing with widely used protocols for small RNA sequencing. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 23% |
| Researcher | 5 | 19% |
| Professor | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Student > Master | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 11 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 35% |
| Chemistry | 2 | 8% |
| Agricultural and Biological Sciences | 2 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Nursing and Health Professions | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 9 | 35% |