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Mendeley readers
Chapter title |
Transient Expression of Viral Proteins in Plants Using Agrobacterium tumefaciens.
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Chapter number | 38 |
Book title |
Vaccine Design
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3389-1_38 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Inga I. Hitzeroth, Albertha R. van Zyl |
Editors |
Sunil Thomas |
Abstract |
Transient expression of viral proteins in plants is a novel alternative to other expression platforms. The viral proteins can be used as potential vaccines or in diagnostics. Nicotiana benthamiana leaves or whole plants are infiltrated with recombinant Agrobacterium that harbor the gene of interest. Protein expression in the plants is rapid and results are obtained within 2-7 days. Here we describe how to make electrocompetent Agrobacterium, how to transform Agrobacterium, how to infiltrate leaves or plants with the recombinant Agrobacterium, and lastly how to extract the protein for analysis by gel electrophoresis. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 1 | 17% |
Researcher | 1 | 17% |
Lecturer > Senior Lecturer | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 33% |
Nursing and Health Professions | 1 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 2 | 33% |