Chapter title |
Detection of Avian Antigen-Specific T Cells Induced by Viral Vaccines.
|
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Chapter number | 5 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Tina Sørensen Dalgaard, Liselotte Rothmann Norup, Helle Risdahl Juul-Madsen |
Editors |
Sunil Thomas |
Abstract |
Live attenuated viral vaccines are widely used in commercial poultry production, but the development of new effective inactivated/subunit vaccines is needed. Studies of avian antigen-specific T cells are primarily based on analyses ex vivo after activating the cells with recall antigen. There is a particular interest in developing robust high-throughput assays as chicken vaccine trials usually comprise many individuals. In many respects, the avian immune system differs from the mammalian, and T cell assessment protocols must be adjusted accordingly to account for, e.g., differences in leukocyte subsets.The carboxyfluorescein succinimidyl ester (CFSE) method described in this chapter has been adapted to chicken cells. In this test, cells of interest are stained with CFSE. The succinimidyl ester group covalently binds to cellular amines forming fluorescent conjugates that are retained in the cells even throughout division. This leads to daughter cells containing half the fluorescence of their parents. When lymphocytes are loaded with CFSE prior to ex vivo stimulation with specific antigen, the measurement of serial halving of its fluorescence by flow cytometry identifies the cells responding to the stimulation. This method has been successfully applied to studies of chicken antigen-specific T cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 29% |
Researcher | 2 | 29% |
Student > Postgraduate | 2 | 29% |
Student > Master | 1 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 43% |
Veterinary Science and Veterinary Medicine | 2 | 29% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Immunology and Microbiology | 1 | 14% |