Chapter title |
Development of Experimental Vaccines Against Liver Flukes.
|
---|---|
Chapter number | 9 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Huan Yong Yap, Peter M. Smooker, Yap, Huan Yong, Smooker, Peter M |
Editors |
Sunil Thomas |
Abstract |
A multitude of experimental vaccines have been developed against liver flukes in the past. However, there has yet to be the development of a commercial livestock vaccine. Reasons for this may be multiple, and include the lack of identification of the best antigen(s), or the immune response induced by those antigens not being appropriate in either magnitude or polarity (and therefore not protective). Cathepsin proteases are the major component of the excretory/secretory (ES) material of liver flukes in all stages of their life cycle in the definitive host and are the primary antigens of interest for the vaccine development in many studies. Hence, this chapter presents the methodologies of using cathepsin proteases as targeted antigens in recombinant protein and DNA vaccine development to engender protective immune responses against fasciolosis.First, the experimental vaccines developed in the past and the criteria of an effective vaccine for fasciolosis are briefly reviewed. Then flowcharts for recombinant protein vaccine and DNA vaccine development are presented, followed by the detailed materials and methodologies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 1 | 14% |
Student > Bachelor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Researcher | 1 | 14% |
Student > Postgraduate | 1 | 14% |
Other | 0 | 0% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 2 | 29% |
Mathematics | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 2 | 29% |