Chapter title |
Vaccine Design
|
---|---|
Chapter number | 7 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Zhang, Yu, Sun, Jing, Wei, Yongwei, Li, Jianrong, Yu Zhang, Jing Sun, Yongwei Wei, Jianrong Li |
Editors |
Sunil Thomas |
Abstract |
Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. aMPV belongs to the family Paramyxoviridae which includes many important human pathogens such as human respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and human parainfluenza virus type 3 (PIV3). The family also includes highly lethal emerging pathogens such as Nipah virus and Hendra virus, as well as agriculturally important viruses such as Newcastle disease virus (NDV). For many of these viruses, there is no effective vaccine. Here, we describe a reverse genetics approach to develop live attenuated aMPV vaccines by inhibiting the viral mRNA cap methyltransferase. The viral mRNA cap methyltransferase is an excellent target for the attenuation of paramyxoviruses because it plays essential roles in mRNA stability, efficient viral protein translation and innate immunity. We have described in detail the materials and methods used to generate recombinant aMPVs that lack viral mRNA cap methyltransferase activity. We have also provided methods to evaluate the genetic stability, pathogenesis, and immunogenicity of live aMPV vaccine candidates in turkeys. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 4 | 20% |
Researcher | 3 | 15% |
Student > Ph. D. Student | 3 | 15% |
Lecturer | 1 | 5% |
Student > Bachelor | 1 | 5% |
Other | 4 | 20% |
Unknown | 4 | 20% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 5 | 25% |
Medicine and Dentistry | 3 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Agricultural and Biological Sciences | 2 | 10% |
Immunology and Microbiology | 1 | 5% |
Other | 2 | 10% |
Unknown | 5 | 25% |