Chapter title |
Strategies for Vaccine Design Using Phage Display-Derived Peptides.
|
---|---|
Chapter number | 28 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Luiz R. Goulart, Paula de S. Santos |
Editors |
Sunil Thomas |
Abstract |
Development of peptide vaccines through the phage display technology is a powerful strategy that relies on short peptides expressed in the phage capsid surface to induce highly targeted immune responses. Phage display-derived immunogenic peptides can be used directly as a phage-fused peptide reagent or as a synthetic peptide with specific modifications, according to target molecule and disease pathogen/parasite. Peptides' selection (mimotopes) can be performed against monoclonal or polyclonal antibodies to disclose determinant regions (epitopes) that can induce a neutralizing response. Validations of mimotopes are performed in vitro and in vivo, based on cell culture and animal models, to demonstrate its immunogenic potential for final vaccine formulations with an appropriate adjuvant. Here we present specific methods for the discovery of novel immunogenic peptides based on phage display. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 15% |
Student > Master | 2 | 15% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 6 | 46% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Veterinary Science and Veterinary Medicine | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 7 | 54% |