Chapter title |
Selection of Vaccine Candidates for Fish Pasteurellosis Using Reverse Vaccinology and an In Vitro Screening Approach.
|
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Chapter number | 12 |
Book title |
Vaccine Design
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Francesca Andreoni, Giulia Amagliani, Mauro Magnani |
Editors |
Sunil Thomas |
Abstract |
The advent of new technologies in recent years has revolutionized the methods by which pathogens are studied and at the same time it has provided new tools to design vaccines against infections for which vaccine development has so far been unsuccessful. The availability of genomic data provides the basis for the reverse vaccinology approach, a biotechnological strategy that uses bioinformatics analysis of microbial genome data for the in silico selection of potential vaccine candidates for the development of protein-based vaccines. The antigens selected by reverse vaccinology can be produced as recombinant proteins and subjected to further in vitro screening assays before in vivo experiments to assess immunogenicity and protection. The reverse vaccinology approach has been applied to several pathogens affecting human health, but also to marine bacteria, including Photobacterium damselae subsp. piscicida causing significant harm in marine aquaculture. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Greece | 1 | 3% |
Unknown | 29 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 20% |
Student > Ph. D. Student | 6 | 20% |
Student > Bachelor | 3 | 10% |
Other | 2 | 7% |
Professor | 1 | 3% |
Other | 0 | 0% |
Unknown | 12 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 20% |
Biochemistry, Genetics and Molecular Biology | 3 | 10% |
Environmental Science | 2 | 7% |
Veterinary Science and Veterinary Medicine | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 1 | 3% |
Unknown | 16 | 53% |