Chapter title |
Vaccine Design: Replication-Defective Adenovirus Vectors.
|
---|---|
Chapter number | 23 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Xiangyang Zhou, Zhiquan Xiang, Hildegund C. J. Ertl |
Editors |
Sunil Thomas |
Abstract |
Replication-defective adenovirus (Ad) vectors were initially developed for gene transfer for correction of genetic diseases. Although Ad vectors achieved high levels of transgene product expression in a variety of target cells, expression of therapeutic proteins was found to be transient as vigorous T cell responses directed to components of the vector as well as the transgene product rapidly eliminate Ad vector-transduced cells. This opened the use of Ad vectors as vaccine carriers and by now a multitude of preclinical as well as clinical studies has shown that Ad vectors induce very potent and sustained transgene product-specific T and B cell responses. This chapter provides guidance on developing E1-deleted Ad vectors based on available viral molecular clones. Specifically, it describes methods for cloning, viral rescue and purification as well as quality control studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 18% |
Student > Doctoral Student | 1 | 9% |
Student > Bachelor | 1 | 9% |
Student > Master | 1 | 9% |
Researcher | 1 | 9% |
Other | 1 | 9% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 18% |
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Business, Management and Accounting | 1 | 9% |
Other | 0 | 0% |
Unknown | 4 | 36% |