Chapter title |
Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control.
|
---|---|
Chapter number | 18 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3389-1_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3388-4, 978-1-4939-3389-1
|
Authors |
Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka |
Editors |
Sunil Thomas |
Abstract |
Ticks (Parasitiformes: Ixodida) are known for their obligate blood feeding habit and their role in transmitting pathogens to various vertebrate hosts. Tick control using chemical acaricides is extensively used particularly in livestock management, but several disadvantages arise from resistance development of many tick species, and concerns on animal product and environmental contamination. Vaccination offers better protection and more cost-effective alternative to application of chemical acaricides, addressing their disadvantages. However, an ideal anti-tick vaccine targeting multiple tick species and all the tick stages is still wanting. Here, we describe the procedures involved in the evaluation of a vaccine candidate antigen against ticks at the laboratory level, from the preparation of recombinant proteins, administration to the rabbit host and monitoring of antibody titer, to tick infestation challenge and determination of the effects of immunization to ticks. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 1 | 13% |
Lecturer | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Researcher | 1 | 13% |
Professor > Associate Professor | 1 | 13% |
Other | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Materials Science | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |